Comparison of rate of islet loss in syngeneic, allogeneic and xenogeneic grafts in rat using quantification of iron oxide labeled islet cells by 3D radial UTE MRI
نویسندگان
چکیده
L. A. Crowe, F. Ris, S. Nielles-Vallespin, P. Speier, M. Kocher, S. Masson, C. Toso, D. Bosco, T. Berney, and J-P. Vallée Department of Radiology, Geneva University Hospital, University of Geneva, Faculty of Medicine, Geneva, Switzerland, Cell Isolation and Transplant Center, Department of Surgery, Geneva University Hospital, Geneva, Switzerland, Siemens AG Medical Solutions, Erlangen, Germany, Biomedical Imaging Group, School of Engineering, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Vaud, Switzerland
منابع مشابه
Characterization of mixed syngeneic-allogeneic and syngeneic-xenogeneic islet-graft rejections in mice. Evidence of functional impairment of the remaining syngeneic islets in xenograft rejections.
Allogeneic mouse islets or xenogeneic rat islets, or fetal porcine islets were implanted under the renal capsule of C57BL/6 mice either alone or carefully mixed with syngeneic islets. With this experimental model the syngeneic islets, although not rejected themselves, are exposed to cytokines and inflammatory mediators released during either allograft or xenograft rejection. No differences in i...
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OBJECT To better understand the fate of islet isografts and allografts, we utilized a magnetic resonance (MR) imaging technique to monitor mouse islets labeled with a novel MR contrast agent, chitosan-coated superparamagnetic iron oxide (CSPIO) nanoparticles. MATERIALS AND METHODS After being incubated with and without CSPIO (10 µg/ml), C57BL/6 mouse islets were examined under transmission el...
متن کاملبررسی اثر مهاری سلولهای بنیادی مزانشیمی بافت چربی بر روی تکثیر سلولهای تک هستهای طحالی موش دیابتی C57BL/6 در محیط آزمایشگاه
Background: Type 1 diabetes (T1D) is a T-cell mediated autoimmune disorder in which pancreas beta-cell destruction causes insulin deficiency and hyperglycemia. In addition to daily insulin treatment, allogeneic islet transplant inT1D is another therapeutic way that needs immunosuppressive drugs to control autoimmune damage and graft rejection. Since life-long application of these drugs is ...
متن کاملImaging islets labeled with magnetic nanoparticles at 1.5 Tesla.
We have developed a magnetic resonance imaging (MRI) technique for imaging Feridex (superparamagnetic iron oxide [SPIO])-labeled islets of Langerhans using a standard clinical 1.5-Tesla (T) scanner and employing steady-state acquisition imaging sequence (3DFIESTA). Both porcine and rat islets were labeled with SPIO by a transfection technique using a combination of poly-l-lysine and electropora...
متن کاملMagnetic resonance imaging of transplanted mouse islets labeled with chitosan-coated superparamagnetic iron oxide nanoparticles.
Although only 10% of islet recipients maintain insulin independence, 80% of them are C-peptide positive at 5 years after transplantation. To better understand the fate of transplanted islets, a magnetic resonance imaging (MRI) technique has been used to detect Feridex-labeled islet grafts in rodents. In this study, we used a novel MRI contrast agent, chitosan-coated superparamagnetic iron oxide...
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